Comparison Research Between Total Error and Uncertainty in Nine Humoral Immune Items / 现代检验医学杂志

ABSTRACT

Objective To evaluate the performance of humoral immune items by the statistical method of total error (TE)and uncertainty.Methods The data of internal quality control were collected by Bio-Rad quality controll software from January to October 2013,and the accumulation means (x-),accumulation standard deviations (s)and accumulation coefficients of var-iation (CV)were computed.According to the external quality assessment plans from Chinese National Center for Clinical Laboratories[2 reports(201311~201315,201321~201325)].The relative bias(bias%)and relative average bias (bias%)of humoral immune item results and target values were calculated.TE was calculated with the accumulation coefficients of vari-ation and the relative bias.By Nordtest method,the uncertainties of humoral immune items were calculated according to the external quality assessment data and internal quality control accumulation CV.Results The maximum TEs with every 2 in-ternal quality control levels of immunoglobulin G (IgG),immunoglobulin A (IgA),immunoglobulin M (IgM),complement 3 (C3 ),complement 4 (C4 ),antistreptolysin“O”(ASO),rheumatoid factor (RF),C-reactive protein (CRP),immunoglobulin E (IgE)were 13.07,14.05,13.99,15.68,14.84,14.,16.55,14.19 ,10.05.The uncertainties of those items were 15.44, 15.82,16.23,17.67,16.73,19.96,19.01,16.25,10.86.In the constituent ratio of the uncertainties,imprecision play major roles in items of C4,CRP,IgE while bias play major roles in items of ASO,RF.The two elements approach parity in items of IgG,IgA,IgM,C3.Conclusion Both TE and uncertainty have good consistency,and they can be the important reference for the analysis and improvement of laboratory quality.But uncertainty can provide more informations for reference.