Effect of different concentrations of pravastatin treatment on macrophage polarity in mice / 重庆医学

ABSTRACT

Objective To study the effect of different concentrations of pravastatin treatment on macrophage polarity in mice .Methods The mice bone marrow sources of macrophages were cultured in vitro ,with the 0μmol/L sodium pravastatin group as control ,by giving 10 ,25 ,50μmol/L sodium pravastatin to conduct the drug intervention for 24 h .The enzyme linked immunosor-bent assay (ELISA) was used to detect the secretion of interleukin 10 (IL-10) and IL-12;the flow cytometry instrument was used to detect cell membrane CD16/32 ,CD206 expression;real time quantitative polymerase chain reaction (RT-qPCR) was adopted to detect toll-like receptor 4 (TLR4) ,myeloid differentiation factor 88 (MyD88) ,interferon regulatory factor 5 (IRF5) mRNA expres-sion .Results After the intervention of pravastatin sodium on macrophages ,as the pravastatin sodium concentration increase ,the expression of IL-12 and CD16/32 was decline ,while the expression of IL-10 and CD206 was risen ,which was accompanied by TLR4 ,MyD88 ,IRF5 mRNA expression down regulatyion ,and a dose dependent manner .Conclusion Sodium pravastatin promote the polarization of macrophages toward an anti-inflammatory macrophage phenotype (M2) ,this effect may be related with the anti-inflammatory effect of sodium pravastatin .