Identification of molecular targets in the response of triple negative breast cancer to chemotherapy / 国际生物医学工程杂志

ABSTRACT

Objective To explore the molecular targets and mechanisms that could influence the sensitivity of triple negative breast cancer (TNBC) to chemotherapy,to provide important references for the chemotherapy based on gene detection.Methods The gene expression dataset GSE43502 was downloaded from the Gene Expression Omnibus in National Center for Biotechnology Information (NCBI).Gene expression data preprocessing was conducted by R Programming.The differentially expressed genes in the TNBC patients that relapsed after chemotherapy compared with the non-relapsed ones were obtained.Besides,the Database for Annotation Visualization and Integrated analysis (DAVID) was used for functional analysis of those genes.The microRNA (miRNA) that might regulate the differentially expressed genes was obtained from the starBase database.Furthermore,Cytoscape was used for the construction of miRNA-gene regulation network.Results Three hundred and twelve differentially expressed genes were obtained.Some biological process related to cell membrane,metal ion homeostasis and pathways involved in immune and inflammatory were found to be enriched in those genes.Furthermore,171 miRNA that might regulate the differentially expressed genes were obtained and 553 miRNA-gene regulation pairs were formed.Conclusions hsa-miR-30d-5p,SIX4,etc.might influence the sensitivity of TNBC to chemotherapy through cell structure or immune system.