Effect of rosuvastatin combined with irbesartan on remodeling of myocar-dial hypertrophy in rats / 中国病理生理杂志

ABSTRACT

[ ABSTRACT] AIM:To evaluate the effect of rosuvastatin combined with irbesartan on the remodeling of myocar -dial hypertrophy in the rats.METHODS:Male SD rats (n=50) were randomly divided into control group, model group, rosuvastatin group , irbesartan group and combination group .The model of myocardial hypertrophy was established by sub-cutaneous injection of isoproterenol at dose of 2.5 mg/kg for 14 d.From the first day of modeling , the rats in control group and model group received intragastrical saline , and the rats in rosuvastatin group , irbesartan group and combination group were treated with rosuvastatin (4 mg· kg-1 · d-1 ), irbesartan (15 mg· kg-1 · d-1 ) and rosuvastatin (4 mg· kg -1 · d-1)+irbesartan (15 mg· kg-1· d-1), respectively.The interventions continued for 4 weeks.After the interventions, the cardiac mass index and left ventricular mass index of the SD rats were measured .Besides, the degree of myocardial hy-pertrophy was observed with HE staining .The mRNA expression of hypertrophy-related factors, such as ANF,β-MHC and AT1R was determined by RT-PCR, and the protein expression of AT1R was determined by Western blot .RESULTS:Compared with control group , the cardiac mass index , left ventricular mass index , as well as the mRNA expression of ANF andβ-MHC in model group were significantly increased (P<0.05).Compared with model group, the above factors in ro-suvastatin group and irbesartan group were decreased ( P<0.05 ) , and the factors in combination group were lower than those in rosuvastatin group and irbesartan group (P<0.05).In addition, the expression of AT1R at mRNA and protein levels in rosuvastatin group and irbesartan group was lower than that in model group ( P<0.05 ) , while the expression AT1R at mRNA and protein levels in combination group was lower than that in rosuvastatin group and irbesartan group (P<0.05).CONCLUSION:Rosuvastatin and irbesartan are equally effective drugs to resist the formation of myocardial hypertrophy by decreasing the expression of AT 1R.Moreover, combination of the 2 drugs is more effective to improve the degree of myocardial hypertrophy than the 2 drugs alone .