Effect of electro-acupuncture combined with sevoflurane anesthesia on regulation of acute stress response in docked tails rats / 重庆医学

ABSTRACT

Objective To investigate the effect of electro‐acupuncture combined with sevoflurane anesthesia on the regula‐tion of acute stress response in docked tails rats .Methods Fifty male SD rats weighing 280-320 g were randomlsy divided into 5 groups(n=10 each)normal group (group Ⅰ) ,model group (group Ⅱ) ,sevoflurane group (group Ⅲ) ,sevoflurane inhalation com‐bined with body acupuncture group (group Ⅳ) and sevoflurane combined with auricular acupuncture group (group Ⅴ) .The groupⅠwas placed in a glass box containing mixture gas with oxygenflow of 30% (flow 2 L/min) without treatment ,the group Ⅱafter docking tail was placed in a glass box containing mixture gas with oxygenflow of 30% (flow 2 L/min) ,the group Ⅲ ,Ⅳ and Ⅴwere placed in the 2% sevoflurane anesthesia box (flow 2 L/min) ,the group Ⅳ was given continuous stimulation at Zusanli (stimu‐lation parameters3 V ,3 Hz ,2 ms) for 30 min per time ,4 times at intervals of 30 min ,the groupⅤ was given the stimulation at Er‐jian with the same stimulation parameters as the groupⅣ .Rats in each group were killed after treatment and the blood at broken neck was collected for measuring serum corticosterone (CORT) and adrenocorticotropic hormone (ACTH) levels by ELISA .The c‐fos protein expression level in hypothalamic tissue was detected by Western blot .Results Compared with the group Ⅰ ,serum ACTH ,CORT levels ,hypothalamic expression of c‐fos protein in the group Ⅱwere significantly increased (P0 .05);the ACTH level in the groupⅤ was significantly decreased compared with the groupⅢ(P<0 .05) and the CORT level in the group Ⅴ was also decreased when compared with the group Ⅳ(P<0 .05) .The c‐fos expression in the groupⅢ ,Ⅳ and Ⅴwere decreased compared with the groupⅡ (P<0 .05) ,there were significant difference among the group Ⅲ ,ⅣandⅤ(P<0 .05) .Conclusion In the treatment of sevoflurane inhalation anesthe‐sia combined with compound electrostimulation at Erjian ,its effect on stress response is superior to simple sevoflurane treatment and sevoflurane combined with compound electrostimulation at Zusanli .The regulation mechanism of electro‐acupuncture combined with inhalation general anesthesia on stress may be related with hypothalamus regulating paraventricular nucleus c‐fos protein se‐cretion and affecting the HPA axis .