Content Determination of Berberine Hydrochloride in Jiangtang Xiaozhi Tablets by HPLC / 中国药房
China Pharmacy
;
(12): 2530-2531,2532, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-605125
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of berberine hydrochloride in the Jiangtang xiao-zhi tablets.METHODS:
HPLC was conducted with the Symmetry C18 column. The mobile phase was acetonitrile-0.05 mol/L sodium dihydrogen phosphate(pH adjusted to 3.0 using phosphoric acid)(24∶76,V/V)at the flow rate of 1.0 ml/min. The detection wave-length was 345 nm,the column temperature was room temperature and injection volume was 10 μl.RESULTS:
The linear range of berberine hydrochloride was 0.522-4.698 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.72%;the average recovery was 97.79%(RSD=2.09%,n=6).CONCLUSIONS:
The method is simple,accurate,sensitive and reproducible,and can be used for the content determination of berberine hydrochloride.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2015
Tipo de documento:
Artigo
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