Content Comparison of 18 Amino Acids in Plancenta Histolysate Determined by Post-column Derivatization Cation-exchange Chromatography and Pre-column Derivatization HPLC / 中国药师
China Pharmacist
; (12): 1830-1833,1846, 2016.
Article
em Zh
| WPRIM
| ID: wpr-605532
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ABSTRACT
Objective:To compare the content differences of 18 amino acids in plancenta histolysate determined by pre-column de-rivatization HPLC and post-column derivatization cation-exchange chromatography ( AARO) . Methods: The HPLC method was per-formed on a C18 column and 2, 4-dinitro chlorobenzene ( DNCB) was used for pre-column derivatization, and then the determination was carried out after adding 0. 1 mol·L-1 borax buffer (pH=9. 1), and the AARO method was used for the direct determination with a strong acid cation-exchange chromatographic column and post-column derivatization. Results: The RSD for reproducibility of the AARO method was 1. 84%-0. 91%, while that of the HPLC method was 1. 87%-1. 04%. Conclusion:Both AARO method and HPLC method can be used for the quantitative determination of 18 amino acids in plancenta histolysate with the similar results. However, pre-column derivatization HPLC method may cause incomplete derivatization and instable derivatives.
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Idioma:
Zh
Revista:
China Pharmacist
Ano de publicação:
2016
Tipo de documento:
Article