Simultaneous Determination of Imperatorin and Isoimperatorin in Qingxuan Tablet by UPLC / 中国药房
China Pharmacy
;
(12): 2983-2984,2985, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-605749
ABSTRACT
OBJECTIVE:
To establish a method for the simultaneous determination of imperatorin and isoimperatorin in Qingxu-an tablet.METHODS:
UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of methanol-water (gradient elution)at a flow rate of 0.3 ml/min,the detection wavelength was 248 nm,column temperature was 30 ℃,and the in-jection volume was 3 μl.RESULTS:
The linear range was 1.305-13.05 μg/ml for imperatorin(r=0.999 9)and 1.096-10.96 μg/ml for isoimperatorin(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recoveries were 97.45%-100.10%(RSD=1.00%,n=9)and 98.33%-101.20%(RSD=1.01%,n=9),respectively.CONCLUSIONS:
The method is accurate,fast and reliable,and can be used for the simultaneous determination of imperatorin and isoimperatorin in Qingxuan tablet.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2016
Tipo de documento:
Artigo
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