Effects of SDF-1 and its receptor CXCR4 on colorectal cancer liver metastasis potential / 中国现代普通外科进展

ABSTRACT

Objective:To investigate chemokine stromal cell derived factor-1 (SDF-1) and its receptor CXCR4 in colon cancer liver metastasis potential effect.Methods:Selected colorectal cancer cell line HT-29,were divided into SDF-1 group(SDF-110 μg/L),SDF-1 +CXCR4 Monoclonal antibody group (10 μg/L SDF-1+20 μg/mL CXCR4) and control group (culture medium),detected SDF-1 and anti CXCR4 monoclonal antibody on HT-29 cell proliferation and migration ability,established nude mouse model of colorectal cancer liver metastasis,detected AMD3100 on nude mice liver metastasis tumor number effect.Results:SDF-1 group in 570nm A value was 0.82 + 0.09,was significantly higher than that of SDF-1+CXCR4 monoclonal antibody group and the control group 0.56 ± 0.10 and 0.53 ± 0.04,the difference was statistically significant (P＜0.05);SDF-1 group of HT-29 cells throughpolycarbonate microporous membrane the number was 156.37 ± 2.11,was significantly higher than that of SDF-1 +CXCR4 monoclonal antibody group and the control group 101.20 ± 28.40 and 97.19 ± 35.39,the difference was statistically significant (P＜0.05);AMD3100 group nude mice liver metastasis tumor number was 6.38 ± 2.11,was significantly lower than that in phosphate buffer solution(PBS) group 18.39 ± 7.59,the difference was statistically significant(P＜0.05).Conclusion:SDF-1and its receptor CXCR4 may be involved in the process of liver metastasis of colon cancer,and its possible mechanism as SDF-1 induced cell proliferation and directional migration.