MicroRNA-10a induces cisplatin resistance by targeting PTEN in human cervical cancer cells / 中国医师杂志

ABSTRACT

Objective To explore the roles of miR-10a in the cisplatin resistance in cervical cancer cell lines,and further study the mechanism.Methods The cells were transfected with the mimics of miR10a and its negative control RNA (NC).Methyl thiazolyl tetrazolium (MTF) assay and fluorescence activated cell sorter were used to analyze drug sensitivity and apoptosis after treating with cisplatin,luciferase reporter assay to identify that miR-10a directly targets phosphatase and tensin homologue deleted on chromosome ten (PTEN).The expression of PTEN gene and its protein levels after tansfection was measured,respectively.Results after transfected with miR-10a mimics:(1) The 50％ inhibition concentration of Hela cells (7.2 μg/ml) was more than in the NC group (5.6 μg/ml).The 50％ inhibition concentration of Siha cells (6.4 μg/ml) was significantly more than in the NC group (3.8 μg/ml) (P＜0.05).(2) The apoptosis rates of Hela cells and Siha cells were significantly lower compared to the NC group (P ＜ 0.05).(3)miR-10a might directly target the 3'-untranslated region (3'-UTR) of PTEN,which significantly changed the PTEN protein level (P ＜ 0.05).Conclusions miR-10a may modulate cisplatin-induced apoptosis in cervical cancer by inhibiting PTEN protein level.