Identification of genomic full-length sequence of human leukocyte antigen-E and its two novel alleles / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 4068-4074, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-606980
ABSTRACT
BACKGROUND:
Human leukocyte antigen-E (HLA-E) is one of non-classical HLA class I genes. Up to now, the polymorphism analysis is mainly aimed at the variation in exon 3 of HLA-E, which determines HLA-E*0101 or HLA-E*0103. However, the identification of the full-length HLA-E and its novel alleles is rare reported.OBJECTIVE:
To establish the method of identification of HLA-E genomic full-length sequence, and to identify its novel alleles in healthy blood donors in Shenzhen, China.METHODS:
Peripheral blood DNA samples were extracted from the subjects, and the amplified primers and sequencing primers in conserved regions were designed according to the sequences of HLA-E published in the IMGT/HLA database.A high-fidelity reaction system was used to amplify the genomic full-length of HLA-E, followed by sequencing,assembling, confirming and typing.RESULTS ANDCONCLUSION:
Herein, we successfully established the method for amplifying genomic full-length sequence and sequence-based typing. Two novel HLA-E alleles were nominated by WHO HLA Nomenclature committee as HLA-E*01010106 and HLA-E*01010107. Compared with the most related allele HLA-E*01010101,HLA-E*01010106 had one nucleotide change at nt-26(G->T) in 5'-promoter, and HLA-E*01010107 had one nucleotide change at nt3345(T->C) in 3'-UTR. The polymorphism data of genomic full-length HLA-E in Chinese individuals need to be filled, and the method we developed here supplies the key technique for the further studies.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2017
Tipo de documento:
Artigo
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