Effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats undergoing one-time exhaustive exercise / 重庆医学

ABSTRACT

Objective To explore the effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats after exhaustive exercise to provide a scientific basis for the prevention of sports injury by Acer truncatum oil.Methods Thirty healthy male SD rats were randomly divided into control group (A,normal diet + saline every day),exhaustive group (B,normal diet + saline daily) and exhaustive with Acer truncatum oil group (C,0.21 mL/kg daily Acer truncatum oil gavage),6 weeks later,the rats performed once exhaustive swimming exercise,then the creatine kinase (CK),lactate dehydrogenase (LDH),cytokine interleukin (IL-1β,IL-10) and tumor necrosis factor alpha (TNF-α) in the serum;malondialdehyde (MDA) homogenate reduction glutathione (GSH),superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-Px) in muscle were measured.Results After exhaustive exercise,when compared with the group A,the serum levels of CK,LDH,IL-1,TNF-α and IL-10 were significantly higher than in the group B(P＜0.05,P＜0.01,P＜0.05,P＜0.01,P＜0.05),IL-10/ TNF-α ratio had no statistically significant difference(P＞0.05),the level of MDA in gastrocnemius muscle increased significantly (P＜0.05),GSH level and GSH-PX activity decreased significantly (P＜0.05,P＜0.01),SOD and CAT activity were not statistically significant (P＞0.05).when compared with the group B,serum CK,LDH level and IL-1 β,TNF-α were significantly decreased (P＜0.05,P＜0.01,P＜0.01,P＜0.05),IL-10 had no statistical significance (P＞0.05),a significant increase in IL-10/TNF-α(P＜0.05),the level of MDA of gastrocnemius muscle decreased significantly (P＜0.05),the level of GSH and SOD,CAT and GSH-PX were significantly increased (P＜0,01,P＜0.01,P＜0.05,P＜0.01).Conclusion Acer truncatum oil can significantly reduce oxidative stress and inflammatory reaction caused by the exhaustive exercise,which has a protective effect on skeletal muscle.