Over-expressed miR-449a inhibitsproliferation and induces apoptosis of SH-SY5Y cells in vitro / 基础医学与临床
Basic & Clinical Medicine
;
(12): 1117-1121, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-608825
ABSTRACT
Objective To explore the effects of miR-449a on the proliferation and apoptosis in human neuroblastoma SH-SY5Y cells.Methods Transferred miR-449a mimics SH-SY5Y cells by LipofectamineTM2000 as experimental group.Cell growth was measured by CCK8 assay,the cell cycle and apoptosis were detected by flow cytometry.Relative expression of c-Myc and Bax/Bcl-2 was detected by Western blot.Results Compare to blank and negative control cells,the proliferation of miR-449a mimics SH-SY5Y cells was decreased(P<0.05).Flow cytometry showed that over-expressed miR-449a resulted in an increase of G0/G1 phase and apoptotic cells.The expression of c-Myc showed significant difference (P<0.05),Expression of pro-apoptotic protein Bax was increased,and that of anti-apoptotic protein Bcl-2 was decreased(P<0.05).Conclusions miR-449a over-expression can inhibit cell proliferation by regulating cell cycles and induce apoptosis mainly through regulating Bcl-2 family proteins expression in SH-SY5Y cells.
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Basic & Clinical Medicine
Ano de publicação:
2017
Tipo de documento:
Artigo
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