Experimental study on phillyrin resist mice immunosuppression induced by Cyclophosphamide / 中国免疫学杂志

ABSTRACT

Objective:To study the effect of phillyrin on immune function in mice treated with cyclophosphamide.Methods: Mice model of immunosuppression was induced by intraperitoneal injection of cyclophosphamide,the mice in the experimental group received phillyrin with 50,100 and 150 mg/kg doses by gavage.While model group and control group received normal salin,all treatments continued 7 d.The thymus and spleen organ coefficients was measured by conventional methods.The phagocytosis of peritoneal macrophages was measured by FITC microspheres.The levels of IL-2,IL-4 and cAMP in peripheral blood were detected by ELISA.The effects of different doses of phillyrin on the above indexes were analyzed in immunosuppressed mice.Results: A mice model of immunosuppression was established after 7 d,the results showed that the immune organ index,phagocytic capacity of macrophages and the level of immune factors in serum could be improved,and the content of cAMP in peripheral blood lymphocytes was decreased in different degree by phillyrin.Conclusion: Phillyrin has the function of immune regulation,which can resist the immunosuppression of cyclophosphamide.