Isolation and Adherent Culture of Neural Stem Cells from Mouse Embryo / 华中科技大学学报(医学版)
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
;
(6): 312-316,340, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-609168
ABSTRACT
Objective To develop a modified method of adherent culturing NSCs to replace the traditional suspension culturing method.Methods Neural stem cells(NSCs)were isolated from brain of fetal mouse at 15.5-day fetal age.In culture bottle which had been pre-coated with poly-L-ornithine hydrochloride/fibronectin(PO/FN),the cells were adherently cultured and passaged.The cell morphology was observed under inverted microscope.NSCs and their differentiated cells were identified by immunofluorescence.Results NSCs obtained in this study were successfully adherently cultured and expressed specific markers.Conclusion NSCs are successfully adherently cultured in this study.As compared with the traditional suspension culturing method,adherent culturing is simpler and has lower wastage of passage.It is easy to observe cell morphology and differentiation by this method.It is also helpful to culturing and storage of NSCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
Ano de publicação:
2017
Tipo de documento:
Artigo
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