Effects of Sulforaphane on Oxidative Stress and Nrf2/HO-1 Signaling Pathway in Renal Tubular Epithelial Cells / 中国药师

ABSTRACT

Objective: To investigate the effects of sulforaphane (SFN) on oxidative stress and Nrf2(Nuclear factor E2-related factor 2)/HO-1(heme oxygenase-1) signaling pathway in renal tubular epithelial cells (HK-2).Methods: HK-2 cells were cultured in vitro and divided into five groups, including the control group, H2O2 group, H2O2+10 μmol·L-1 SFN group, H2O2+20μmol·L-1 SFN group and H2O2+40 μmol·L-1 SFN group.The levels of MDA, NO, GSH and the activity of SOD were measured.The expression levels of Nrf2, HO-1 mRNAR and proteins were analyzed using RT-PCR and Western Blot methods.Results: The levels of MDA and NO in H2O2 group significantly increased when compared with those in the control group, while the levels of GSH and the activity of SOD significantly decreased(P<0.05).The levels of MDA and NO in the cells after the treatment of sulforaphane at different concentrations significantly reduced when compared with those in H2O2 group, while the levels of GSH and the activity of SOD significantly increased(P<0.05).The results of RT-PCR and Western Blot analysis showed that the expression levels of Nrf2, HO-1 mRNAR and proteins in the cells after the treatment of sulforaphane at different concentrations were significantly up-regulated when compared with those in H2O2 group (P<0.05).Conclusion: Sulforaphane can strengthen the antagonism effects on oxidative stress injury in HK-2 cells and the underlying mechanism might be associated with activating Nrf2/HO-1 signal transduction pathway.