Analysis of the changes of serum IL-6 and TNF-α levels in rats with oral ulcer induced by modified red powder and vitamin B12 / 重庆医学

ABSTRACT

Objective To investigate the therapeutic effect of modified powder and vitamin B12 on the treatment of recurrent oral ulcer in rats and its effect on serum interleukin-6 (IL-6) and tumor necrosis factor alpha(TNF-α).Methods Forty-eight male Sprague-Dawley (SD) rats were randomly divided into normal group (equal distilled water),model group (equal distilled water),positive control group (levamisole 20 mg/kg gavage) and experimental group (containing dcsgd crude drugs 1 g/mL,1.278 mL/200 g+ B12 20 g/kg gavage),each group with 12 rats.Model group,positive control group and experimental group were treated with immunological method to establish the recurrent oral ulcer mode.Each group treated with the corresponding treatment methods for 20 days.Results The oral ulcer number,duration time in positive control group and the experimental group were significantly lower than that in model group rats(P＜0.05),ulcer interval time was significantly longer than the rats in the model group (P＜0.05);the serum IL-6,TNF-α detection level in positive control group and experimental group were significantly lower than in the model group(P＜0.05);serum IL-6,TNF-α detection level in model group were significantly higher than those in normal group(P＜0.05);the peripheral blood SOD,GSHPx levels in positive control group and experimental group rats were significantly higher than that in model group(P＜0.05),the level of MDA was significantly lower than that in model group (P＜0.05).Conclusion Modified powder and vitamin B12 have therapeutic effects on recurrent oral ulcer in rats.The mechanism may be related to reducing the inflammatory reaction and improving the antioxidant capacity of the tissue.