Study of interference and influence of blood specimen hemolysis in biochemical test results / 中国生化药物杂志

ABSTRACT

Objective To study the interference and influence of blood specimen hemolysis in biochemical test results.Methods80 cases of personnel received physical examination in our hospital from May 2014 to May 2016 were selected, these personnel were divided into hemolytic group (n=40) and non hemolytic group (n=40) on the basis of blood specimen hemolysis occurred situations, 5mL morning fasting venous blood of the two groups was collected, they were placed in heparinized tubes, the blood of the hemolytic group was stirred by bamboo inducing hemolysis;The blood of the non hemolysis group was without the above treatment, directly at the rate 2500r/min 10min centrifugal separating serum, the K+, GLU, ALB, TP, CK, TBIL, BUN, ALT, AST, LDH levels of the two groups were detected by automatic biochemical reaction analyzer, and then the biochemical indicators levels of the two groups were statistically analyzed.ResultsThe level of GLU in the hemolysis group was significantly lower than that in the non hemolytic group (P<0.05), the K+, TP, CK, TBIL, AST, LDH levels in the hemolysis group were significantly higher than those in the non hemolytic group (P<0.05), but the differences of ALB, BUN, ALT levels between the two groups were not significant.ConclusionBlood specimen hemolysis can interfere and influence the biochemical tests results to some extent.