Preparation of specimen about mouse liver tissue for scanning electron microscope and its improvement method / 中国医学装备
China Medical Equipment
;
(12): 165-167, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-612756
ABSTRACT
Objective:
To explore and optimize the preparation method of specimen about mouse liver tissue for scanning electron microscopy.Methods:
The specimens of mouse liver using for scanning electron microscope were prepared through improving the fixed location of perfusion, optimizing material and procedure of perfusion, adopting fixation of perfusion via portal vein and changing the process of fixation and so on. And the results of morphological observation of histocyte were compared between the improved method and traditional method.Results:
The improved preparation method could save the liver tissue and the living state of the cells in preferably situation. By using this method, the ultra-micro morphological structure of liver tissue and cell could be clearly observed and the ideal effect of observation could be achieved. Besides, its operation was simple and its success rate was high. Therefore, it was superior to traditional method.Conclusion:
The preparation of specimen about the fixation of mouse liver tissue via perfusion of portal vein for scanning electron microscope is a superior method, which has certain applied value.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Medical Equipment
Ano de publicação:
2017
Tipo de documento:
Artigo
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