Study on effect and mechanism of GSPB2 for protecting vascular endothelial cell and delaying apoptosis / 重庆医学

ABSTRACT

Objective To observe the protective effect of GSPB2 for delaying the human endothelial cells apoptosis with H2O2 induced human endothelial cells apoptosis as the model and to investigate its mechanism.Methods The human endothelial cells were pre-treated by 0.5 mmol H2O2,then different concentrations of GSPB2(5.0,10.0,20.0 μmol/L) was added for protecting cells.The cellular apoptosis was detected by TUNEL method;the apoptosis related molecules,such as Caspase-3,Bax,Bcl-2,and influencing apoptosis related molecules such as PI3K,p-Akt and Akt protein expression were detected by Western bl0t;Transwell chamber was used to detect cell migration situation.Results 0.5 mmol H2O2 induced obvious increase of human endothelial cells (P＜0.01).GSPB2 could significantly alleviate the H2O2 induced apoptosis of human endothelial cells(P＜0.05).10 μmol/mL GSPB2 could be close to return to H2O2 untreated control group level.Further found that Caspase-3,Bax protein expression were up-regulaed,Bcl-2 expression was down-regulated and the expression of PI3K,p-Akt and Akt wa sup-regulated.Conclusion The different concentrations of GSPB2 has a protective effect on vascular endothelial cells induced by H2O2,moreover which manifested by the concentration and time dependence.Its mechanism is related with the related molecules PI3K,p-Akt and Akt