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Suppression of VEGF and Decrease in Vascular Leakage by Pyridoxal 5'-Phosphate in Diabetic Rats
Journal of the Korean Ophthalmological Society ; : 700-706, 2012.
Artigo em Coreano | WPRIM | ID: wpr-61431
ABSTRACT

PURPOSE:

The authors of the present study investigated whether pyridoxal 5'-phosphate (PLP), an active coenzyme of vitamin B6, could inhibit the development of diabetic retinopathy in streptozotocin (STZ)-induced diabetic rats.

METHODS:

Seven-week-old Spraque-Dawley rats (n = 20) were used in the present study. STZ (70 mg/kg) was injected intraperitoneally to induce diabetes. Blood glucose and body weight were monitored. Intraperitoneal injections of 5 microg and 50 microg PLP were administered every two days from the second week of induced diabetes. During the third week of PLP injections, the concentration level of plasma homocysteine was measured. In addition, functional status was examined by vitreous fluorophotometer and anatomical status by vascular endothelial growth factor (VEGF) staining in the retina.

RESULTS:

Based on vitreous fluorophotometry examination, the PLP injection group proved to have a lower level of fluorescein concentration in the vitreous. Additionally, immunohistochemical staining revealed down-regulation of VEGF expression in the PLP group. In addition, the PLP group had a lower plasma homocysteine concentration. However, an over-dosage injection of PLP did not appear to have any noticeable impact on the treatment of diabetes.

CONCLUSIONS:

PLP, an active coenzyme of vitamin B6, proved to have inhibitory effects on VEGF expression and vascular leakage in the diabetic rat retina.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasma / Piridoxal / Retina / Glicemia / Peso Corporal / Regulação para Baixo / Fluorofotometria / Estreptozocina / Fluoresceína / Vitamina B 6 Limite: Animais Idioma: Coreano Revista: Journal of the Korean Ophthalmological Society Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasma / Piridoxal / Retina / Glicemia / Peso Corporal / Regulação para Baixo / Fluorofotometria / Estreptozocina / Fluoresceína / Vitamina B 6 Limite: Animais Idioma: Coreano Revista: Journal of the Korean Ophthalmological Society Ano de publicação: 2012 Tipo de documento: Artigo