Study on the correlation between CD4+ CD25+ Tr cells and IL-17 levels in peripheral blood of patients with antiphospholipid syndrome / 中国基层医药

ABSTRACT

Objective To explore the changes of Tr cells and IL-17 levels in peripheral blood of patients with antiphospholipid syndrome (APS) and the clinical significance.Methods The quantity of the CD4+ CD25+ Tr cells and proportion of CD4+ CD25+ Tr cells/CD4+ T cells were detected by flow cytomety,and the levels of IL-17 were detected by ELISA in 60 cases with APS(APS group) and 60 healthy people(control group).Results The levels of Tr,Tr/CD4+,Foxp + 3 Tr/Tr in the APS group were (3.03 ± 0.40) ％,(0.11 ± 0.02),(0.40 ± 0.10),respectively,which in the control group were (5.76 ± 0.84)％,(0.15 ± 0.03),(0.73 ± 0.15),respectively,the differences were statistically significant(t =22.73,8.59,14.18,all P ＜ 0.01).The levels of serum IL-17 and IL-17/Tr cells in APS group were (15.31 ±2.00)pg/mL,(5.05 ±0.29)pg/mL,respectively,which in the control group were (6.63 ± 1.52) pg/mL,(1.15 ± 0.14) pg/mL,respectively,there were statistically significant differences (t =29.17,93.81,all P ＜0.05).The levels of serum IL-17 was negatively correlated with Tr cells level in peripheral blood of patients with APS(r =-0.801,P ＜0.01).Conclusion There is peripheral blood cellular immune function disorder in APS patients,the number and active changes of CD4+ CD25+ Tr cells and IL-17/Tr ratio imbalance may play a role in the pathogenesis of APS.