Effect of negative-pressure wound therapy on adipose-derived stem cells migration behavior / 重庆医学

ABSTRACT

Objective To investigate the effect of negative-pressure wound therapy (NPWT) on the migration behavior of rat adipose-derived stem cells (ADSCs).Methods Eight (20 ±-2)kg Bama miniature pigs respectively received the sham operation (group A,n=16),continuous negative pressure suction (group B,n =8) and intermittent negative pressure suction (group C,n =8).The pigs were observed for 3 months after 72 h treatment.The effect of NPWT on the migration behavior of ADSCs was observed at the cellular level.In order to avoid the influence of serum-free on cell viability,ADSCs were extracted and treated with continuous negative pressure suction or intermittent negative pressure suction for 8 h.The migration ability of ADSCs was detected by transwell.The proliferation ability of ADSCs was detected by MTT.The expression of hypoxia inducible factor-1α (HIF-1α) was detected by Western blot.Results The wound healing effect of continuous negative pressure suction group and intermittent negative pressure suction group was better than that of the control group,and compared with the control group,the number of migrating ADSCs cells had statistically significant difference (P＜0.05,P＜0.01),but cell proliferation had no statistically significant difference.The expression of HIF-1α was consistent with the migration results of ADSCs.Conclusion NPWT can promote the migration of rat ADSCs by up-regulating HIF-1α protein expression.