Detection of aaP, aggR, and astA in strains of ETEC, EPEC, EIEC and non-diarrheagenic E.coli

ABSTRACT

Background: aaP, aggR, and astA have been found to play important roles in diarrheal pathogenecity of EAEC. They may be exist in other diarrheagenic E.coli (DEC). Objectives: To determine the distribution of aaP, aggR, and astA in ETEC, EPEC, EIEC and non-diarrheagenic E.coli. Subjects and method: 75 strains of ETEC, EPEC, EIEC and 100 non-DEC have been screen by PCR with primers specific toaaP, aggR, and astA. Results: aaP, aggR, and astA have been seen in DEC with the prevence from 7 to 72,7%. The highest prevence was in EIEC, 72,7% for aap; 45,5% in EIEC for aggR; and 50% in ETEC for astA. 14% of non-DEC harbored aggR and more than 30% harbored aap and astA. Conclusion: This finding has contributed to understanding the distribution of aap, aggR and astA in ETEC, EPEC, EIEC and non-DEC as well.