Accurate determination of HLA ambiguous results based on group-specific haploid full-length sequencing / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 3208-3215, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-616350
ABSTRACT
BACKGROUND:
Due to the polymorphism of HLA, a large number of ambiguities have been generated by conventional HLA typing techniques, and confirmed stereotypes of ambiguous results based on group-specific haploid full-length typing are rarely reported.OBJECTIVE:
To analyze the accuracy of HLA-typing ambigulity based on group-specific haploid full-length sequencing.METHODS:
The low-resolution results were used as the starting point for two ambiguous samples. Sanger sequencing (PCR-SBT) based on haploid full-length was performed after group-specific amplification. RESULTS ANDCONCLUSION:
One case showed a new A*020301 allele, which was found a mutation in NT817 from C to T in comparison with A*11010101. The other case indicated another new C*07020101, which was found a mutation in NT879 from A to G in comparison with C*080101. In conclusion, these results indicate that the group-specific haploid full-length sequencing method can be used to accurately classify HLA alleles and to discover new alleles.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2017
Tipo de documento:
Artigo
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