Effect of Banxia Xiexin Decoction on Expression of TLRs/NF-κB Pathway Related Factors in Colon Tissue of Rats with Ulcerative Colitis / 中国药房

ABSTRACT

OBJECTIVE:To observe the effect of Banxia Xiexin decoction on nuclear transcription factor κB p65 (NF-κB p65),NF-κB inhibitory protein α(IκB-α)and Toll-like receptor 4(TLR4)in colon tissue of rats with ulcerative colitis(UC),and explore the possible mechanism for UC treatment. METHODS:Rats were randomly divided into normal group (normal saline), model group (normal saline),Sulfasalazine enteric coated tablet (SASP,positive group,0.3 g/kg),Banxia Xiexin decoction low-dose,medium-dose,high-dose groups (3.9,7.8,11.7 g/kg),8 in each group. Except for normal group,other groups were used trinitrobenzene sulfonic acid-ethanol method to reduce UC model. After modeling,they were administrated,ig,once a day, for 3 weeks. After administration,NF-κB p65,IκB-α,TLR4 mRNA and protein expressions in colon tissue of rats in each group were detected. RESULTS:Compared with normal group,NF-κB p65,IκB-α,TLR4 mRNA and protein expressions in colon tissue of rats in other groups were significantly increased(P<0.05). Compared with model group,NF-κB p65,IκB-α,TLR4 mRNA and protein expressions in colon tissue of rats in each administration group were significantly decreased (P<0.05);showing certain dose-dependent,and the decreasing degree of above indexes in colon tissue of rats in Banxia Xiexin decoction high-dose group were higher than SASP group (P<0.05). CONCLUSIONS:Banxia Xiexin decoction can down-regulate the NF-κB p65,IκB-α, TLR4 mRNA and protein expressions in colon tissue of UC rats,which may be one of the mechanisms for UC treatment.