Preparation and characterization of chitosan/hyaluronic acid nanoparticles for microRNA-148b delivery / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 31-35, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-619225
ABSTRACT
Objective:
To prepare and characterize chitosan/hyaluronic acid (CS/HA) nanoparticles for miRNA-148b (miR-148b)delivery.Methods:
CS/HA/miR-148b nanoparticles were prepared.The particle size,zeta potential,morphology were investigated by nano laser granulometer and TEM respectively.The encapsulation efficiency of CS/HA nanoparticles was evaluated by gel retarding analysis.The cytotoxicity and transfection efficiency of CS/HA/miR-148b nanoparticles on rat bone marrow mesenchymal stem cells (MSCs) were evaluated by CCK-8 assay,transfection assay and FCM respectively.Results:
The CS/HA/miR-148b nanoparticles were discrete spherical particles with the diameter of 160 to 370 nm and zeta potential of + 15 to +41 mV.N/P ratio at 20∶1 of the nanoparticles showed the optimum encapsulation efficiency for miR-148b.CS/HA/miR-148b nanoparticles exhibited no cytotoxicity to MSCs and could deliver miR-148b to the MSCs at high transfection efficiency.Conclusion:
CS/HA/miR-148b nanoparticles are safe and effective for miR-148b delivery into MSCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Practical Stomatology
Ano de publicação:
2017
Tipo de documento:
Artigo
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