The effects of platelet-rich fibrin extract on human periodontal ligament cells stimulated by TNF-α in vitro / 实用口腔医学杂志

ABSTRACT

Objective:To investigate the effects of platelet-rich fibrin extract (PRFe) on the osteogenetic differentiation and mineralization of human periodontal ligament cells (hPDLCs) stimulated by tumor necrosis factor-α (TNF-α) in vitro.Methods:hPDLCs were cultured and identified.PRFe was obtained by Choukroun's protocols.The cells were treated as the 4 groups① the blank control group,②TNF-α(10 ng/ml),③ PRFe and ④PRFe + TNF-α(10 ng/ml) respectively.Alkaline phosphatase activity was detected by the ALP kit.The alizarin red dye was used to observe the mineralization of the cells.Runx2 and Osterix expression was quantified by Western blotting.Results:The ALP activity,mineralization level and the expression of Runx2 and Osterin of the TNF-oα group were lower that those of the control group(P ＜ 0.05).The examined indexs of PRFe group were higher than that of the control group(P ＜0.05).The indexs of the PRFe +TNF-α group were higher than that of TNF-α group(P ＜0.05).The indexs of PRFe group were higher than that of PRFe + TNF-α group(P ＜ 0.05).Conclusion:PRFe may promote the osteogenetic differentiation of hPDLCs stimulated by TNF-α in vitro.