Ectopic Expression of Caveolin-1 Induces COX-2 Expression in Rabbit Articular Chondrocytes via MAP Kinase Pathway
Immune Network
;
: 123-127, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-61958
ABSTRACT
BACKGROUND:
Caveolin-1 is a principal component of caveolae membranes in vivo. Although expression of caveolae structure and expression of caveolin family, caveolin-1, -2 and -3, was known in chondrocytes, the functional role of caveolae and caveolins in chondrocytes remains unknown. In this study, we investigated the role of caveolin-1 in articular chondrocytes.METHODS:
Rabbit articular chondrocytes were prepared from cartilage slices of 2-week-old New Zealand white rabbits by enzymatic digestion. Caveolin-1 cDNA was transfected to articular chondrocytes using LipofectaminePLUS. The cyclooxygenase-2 (COX-2) expression levels were determined by immunoblot analysis, immunostaining, immunohistochemistry, and prostaglandin E2 (PGE2) assay was used to measure the COX-2 activity.RESULTS:
Ectopic expression of caveolin-1 induced COX-2 expression and activity, as indicated by immunoblot analysis and PGE2 assay. And also, overexpression of caveolin-1 stimulated activation of p38 kinase and ERK-1/ -2. Inhibition of p38 kinase and ERK-1/-2 with SB203580 and PD98059, respectively, led to a dose-dependent decrease COX-2 expression and PGE2 production in caveolin-1-transfected cells.CONCLUSION:
Taken together, our data suggest that ectopic expression of caveolin-1 contributes to the expression and activity of COX-2 in articular chondrocytes through MAP kinase pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Imuno-Histoquímica
/
Dinoprostona
/
Cartilagem
/
DNA Complementar
/
Condrócitos
/
Cavéolas
/
Caveolinas
/
Digestão
/
Ciclo-Oxigenase 2
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2006
Tipo de documento:
Artigo
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