Effects of Psoralen on Alcohol-induced Proliferation of Osteoblasts / 广州中医药大学学报

ABSTRACT

Objective To observe the effect of psoralen on the proliferation of osteoblasts induced by alcohol, thus to explore the mechanism of psoralen in preventing and treating alcohol-induced osteoporosis. Methods The osteoblasts separated from neonatal rat skull were identified by alkaline phosphatase (ALP) staining method. The in-vitro confirmed osteoblasts were randomly divided into 4 groups, namely blank control group, alcohol group (model group), psoralen group, and psoralen plus alcohol group. The alcohol-induced osteoblast proliferation in various groups was determined by methyl thiazolyl tetrazolium (MTT) method. Results Compared with the blank control group, the alcohol-induced osteoblast proliferation at culturing hour 24, 48, 72, 96 in alcoholgroup was significantly decreased (P0.05). Compared with the alcohol group, osteoblast proliferation at culturing hour 24 in psoralen plus alcohol group was significantly increased (P 0.05). Conclusion Psoralen has certain effect on promoting alcohol-induced osteoblast proliferation in vitro.