Detection of Avanafil and Flibanserin in Health Food by HPLC-MS/MS / 医药导报

ABSTRACT

Objective To develop a method for detection of avanafil and flibanserin adulterated in health food by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods The separation and analysis were performed on an Agilent Eclipse Plus C18 column(2.1 mm×100 mm,1.8 μm),with a mobile phase of acetonitrile and 0.1%acetic acid(containing 20 mmol·L-1 ammonium acetate)(6040).Electrospray ionization(ESI) source was applied and operated in positive mode.Multiple reaction monitoring(MRM) mode was used to quantify avanafil and flibanserin.Results The assay linearity of avanafil and flibanserin were confirmed in the range of 2-20 ng·mL-1(r2>0.99).The extraction recoveries varied from 94.6% to 110.0%,and the precision of RSD was <5.0%.The limits of detection were 0.21 and 0.42 μg·kg-1 for avanafil and flibanserin,respectively.Conclusion The method was specific,sensitive and accurate.Therefore,it can be used to detect avanafil and flibanserin which were illegally added in health food.