Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach
The Korean Journal of Parasitology
;
: 343-346, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-62038
ABSTRACT
The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parasitologia
/
Caramujos
/
Tailândia
/
Reação em Cadeia da Polimerase
/
Prevalência
/
Primers do DNA
/
DNA de Helmintos
/
Heterophyidae
/
Microscopia
Tipo de estudo:
Estudo de prevalência
/
Fatores de risco
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2010
Tipo de documento:
Artigo
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