Molecular Cloning of Plasmodium vivax Calcium-Dependent Protein Kinase 4
The Korean Journal of Parasitology
; : 319-324, 2010.
Article
em En
| WPRIM
| ID: wpr-62043
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WPRO
ABSTRACT
A family of calcium-dependent protein kinases (CDPKs) is a unique enzyme which plays crucial roles in intracellular calcium signaling in plants, algae, and protozoa. CDPKs of malaria parasites are known to be key regulators for stage-specific cellular responses to calcium, a widespread secondary messenger that controls the progression of the parasite. In our study, we identified a gene encoding Plasmodium vivax CDPK4 (PvCDPK4) and characterized its molecular property and cellular localization. PvCDPK4 was a typical CDPK which had well-conserved N-terminal kinase domain and C-terminal calmodulin-like structure with 4 EF hand motifs for calcium-binding. The recombinant protein of EF hand domain of PvCDPK4 was expressed in E. coli and a 34 kDa product was obtained. Immunofluorescence assay by confocal laser microscopy revealed that the protein was expressed at the mature schizont of P. vivax. The expression of PvCDPK4-EF in schizont suggests that it may participate in the proliferation or egress process in the life cycle of this parasite.
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Assunto principal:
Plasmodium vivax
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Proteínas Quinases
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Dados de Sequência Molecular
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Expressão Gênica
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Proteínas de Protozoários
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Alinhamento de Sequência
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Sequência de Aminoácidos
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Clonagem Molecular
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Estrutura Terciária de Proteína
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Sequência Conservada
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2010
Tipo de documento:
Article