Biological Titer Determination of Traditional She Medicine-Gardeniae Radix / 中国药师
China Pharmacist
; (12): 1463-1465, 2017.
Article
em Zh
| WPRIM
| ID: wpr-621141
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WPRO
ABSTRACT
Objective: To study the antimicrobial effectiveness against traditional She medicine-Gardeniae Radix and determine the titer of Gardeniae Radix.Methods: The antibacterial activity of five standard strains(Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , Candida albicans and Aspergillus niger)was investigated.A method for the biological value evaluation of Gardeniae Radix was established by using a turbidity method, and compared with the content of chikusetsu saponin Ⅳa determined by HPLC.Results: The sensitivity of Gardeniae Radix to Staphylococcus aureus and Escherichia coli was high.The recovery of the turbidity method was within the range of 90%-110%, and the reliability limit rate was less than 5%.The content of chikusetsu saponin Ⅳa in Gardeniae Radix was not completely consistent with the result of the bioassay method.Conclusion: The bioassay method established in the experiment can be one of the methods for the quality evaluation of Gardeniae Radix.
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Índice:
WPRIM
Idioma:
Zh
Revista:
China Pharmacist
Ano de publicação:
2017
Tipo de documento:
Article