Effects of PYY on the interdigestive migrating myoelectric complex in the small intestine in vivo and the neural and endocrinal mechanisms of the effects / 药物分析学报

ABSTRACT

Objective To investigate the effects of peptide YY (PYY) on the interdigestive migrating myoelectrlc complex (MMC) in the small intestine in vivo and explore the neural and endecrinal mechanisms of the effects. Methods Spragne-Dawley rats were supplied with a venous catheter and bipolar electrodes in the duodenum and jejunum for electromyography of stomach and small intestine in wake state. PYY, phentolamine, nitro-L-arginine (L-NNA, the inhibitor of nitric oxide synthase) and atropine were served with PYY respectively. The plasma motilin levels before and after the infusion of PYY were observed. Results At all the three recording points, PYY lengthened the drde length of MMC [from (591.90±128.98)s to (999.25±216.59)s, P<0. 01] and lowered the frequency of phase Ⅲ [from (39.28±8.40) min-1 to (22.08±3.13) min-1 , P<0.01], amplitude of phase Ⅲ [from (0. 320±0.060)mV to (0. 179±0.030)mV, P<0.01], and the portion of phase Ⅲ over the whole circle length [from (28. 61 ± 5.84)% to (15.43 ±5.16)% , P<0.01]. Phentolumine had no influence on the role of PYY. Administered L-NNA combined with PYY, the percentage of phase Ⅲ increased [(42. 09±8.67)%] compared with that of control(P<0.01) and compared with that of PYY administered alone (P<0. 01) too. Atropine combined with PYY showed stronger depressing effects on MMC. No significant difference was found between the plasma motilin levels before and after the infusion of PYY. Conclusion PYY my inhibit the interdigestive intestine motility through the none-adrenergic none-choUnergic tract, while the m-receptor tract and circulating motilin are probably not involved In the depressing effect.