THE CLONING OF HUMAN NEUROTROPHIN-3 GENE / 药物分析学报
Journal of Pharmaceutical Analysis
; (6): 8-13, 1999.
Article
em Zh
| WPRIM
| ID: wpr-621895
Biblioteca responsável:
WPRO
ABSTRACT
In the present study, we have cloned the gene of human neurotrophin-3 (hNT-3) from the genomic DNA of white blood cells (WBC) by polymerase chain reaction (PCR). The amplification products were cloned into pUC19 and sequenced. Genomic sequence comparison of the cloned fragment and the reported hNT-3 (GenBank M61180) reveals 7 base differences: 1 in the signal peptide, 3 in the prepro peptide, and 3 in the mature hNT-3. Except the 2 varied bases (16th, T to G; 285th, A to C) in the signal peptide and pro-sequence resulted in the change of their encoded amino-acids (Tyr→Asp; Gln→His), the other varied bases have no influence on their respective encoded amino-acids, and all the changes have no influence on the open reading frame (ORF) of the hNT-3.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Analysis
Ano de publicação:
1999
Tipo de documento:
Article