Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period
Asian Spine Journal
;
: 329-336, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-62208
ABSTRACT
STUDY DESIGN:
Controlled laboratory study.PURPOSE:
This study aimed to evaluate the efficacy of platelet-rich plasma (PRP) stored at room temperature (RT), frozen, or after freeze-drying. OVERVIEW OF LITERATURE PRP enriches tissue repair and regeneration, and is a novel treatment option for musculoskeletal pathologies. However, whether biological activity is preserved during PRP storage remains uncertain.METHODS:
PRP was prepared from blood of 12 healthy human volunteers (200 mL/person) and stored using threemethods:
PRP was stored at RT with shaking, PRP was frozen and stored at −80℃, or PRP was freeze-dried and stored at RT. Platelet counts and growth factor content were examined immediately after preparation, as well as 2, 4, and 8 weeks after storage. Platelet activation rate was quantified by flow cytometry.RESULTS:
Platelet counts were impossible to determine in many RT samples after 2 weeks, but they remained at constant levels in frozen and freeze-dried samples, even after 8 weeks of storage. Flow cytometry showed approximately 80% activation of the platelets regardless of storage conditions. Almost no growth factors were detected in the RT samples after 8 weeks, while low but significant expression was detected in the frozen and freeze-dried PRP. Over time, the mean relative concentrations of various growth factors decreased significantly or disappeared in the RT group. In the frozen group, levels were maintained for 4 weeks, but decreased significantly by 8 weeks (p <0.05). The freeze-dried group maintained baseline levels of growth factors for the entire 8-week duration.CONCLUSIONS:
Freeze-drying enables PRP storage while maintaining bioactivity and efficacy for extended periods.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Contagem de Plaquetas
/
Regeneração
/
Preservação de Sangue
/
Ativação Plaquetária
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Plasma Rico em Plaquetas
/
Voluntários Saudáveis
/
Citometria de Fluxo
/
Liofilização
Limite:
Humanos
Idioma:
Inglês
Revista:
Asian Spine Journal
Ano de publicação:
2017
Tipo de documento:
Artigo
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