Preparation and identification of polyclonal antibody against protein H1b: the variant of major subunit of human ASGPR / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
;
(12): 917-919, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-622307
ABSTRACT
AIM:
To prepare and identify mouse polyclonal antibody against protein Hlb, which is the variant of major subunit of human ASGPR.METHODS:
Hlb specific peptide was synthesized and coupled with keyhole limpet hemocyanin (KLH) for immunization. Then H1b-KLH conjugation was injected into mouse subcutaneously to produce polyclonal antibody. ELISA assay was used to detect the titer of the antibody. Antibody was also identified by Western blot and immunohistochemistry assays.RESULTS:
Mouse antibody against Hlb was prepared after injection of H1bKLH conjugation. The titer of H1b antibody was about 110~5.Western blot confirmed its high specificity. This antibody could also be used for immunohistochemistry analysis.CONCLUSION:
The successful preparation of the polyclonal antibody against protein H1b, which can discriminate the two variants of the major subunit of ASGPR with high specificity, will provide an efficient reagent for further study of the physiologic functions of H1b and its role in the pathogenesis of human disease.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Cellular and Molecular Immunology
Ano de publicação:
2009
Tipo de documento:
Artigo
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