Detection of aap, aggR, and astA in strains off enteroaggregative E.Coli

ABSTRACT

Background: Aap, aggR, astA have been found to play important roles in diarrheal pathogenecity of enteroaggregative (EAEC). Objective: (1) To determine the distribution of aap, aggR, astA in EAEC. (2) To compare the distribution of aap, aggR, astA in EAEC isolated from healthy children and children with diarrhea. Subject and methods: \r\n', u'86 strains of EAEC isolated from children under 5 living in Hanoi, have been screened by PCR with specific primers. Results: Aap was found at the highest prevalence of 96.5%, aggR (79.1 %) and astA (60.5%). 37.2% of strains had all of aap, aggR and astA. None of strains was shown negative with these genes. Distributive rate of aap, aggR, astA in EAEC strains isolated from children with diarrhea was higher than EAEC strains isolated from healthy children. However, this difference was not statistically significant. Nearly 100% of the EAEC strains isolated from children with diarrhea had aap gene. The rate of aggR and astA was lower. Among children aged 0-24 months and children aged 25-60 months, distribution of these three genes was not different with statistical significance Conclusion: This finding has contributed to understanding the distribution of aap, aggR and astA of EAEC\r\n', u'\r\n', u'