Strategy for generating tissue-engineered human bone construct
The Medical Journal of Malaysia
;
: 43-44, 2004.
Artigo
em Malaiala
| WPRIM
| ID: wpr-629925
ABSTRACT
The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas de Cultura de Órgãos
/
Periósteo
/
Regeneração Óssea
/
Divisão Celular
/
Colágeno
/
Transplante Ósseo
/
Engenharia Tecidual
/
Transplante de Células-Tronco Mesenquimais
Tipo de estudo:
Estudo prognóstico
Idioma:
Malaiala
Revista:
The Medical Journal of Malaysia
Ano de publicação:
2004
Tipo de documento:
Artigo
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