Analytical and diagnostic performance of an automated anti-CCPassay
The Malaysian Journal of Pathology
;
: 101-106, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-630050
ABSTRACT
Aim:
Autoantibodies against cyclic citrullinated peptide (anti-CCP) are considered to be a sensitive and specifi c marker for rheumatoid arthritis (RA). This study evaluated the diagnostic and analytical performances of the automated anti-CCP assay. Materials andMethod:
Sera from 80 patients with established RA, 65 from other rheumatic diseases (non-RA) and 55 from healthy controls were studied using second generation anti-CCP. Rheumatoid factor (RF) was also assayed in each sample, and the results were compared to the anti-CCP fi ndings. Serum pools were used to determine the precision and linearity.Results:
At a cut-off of 7.4 U/ml for anti-CCP, the sensitivity and specifi city for RA were 65% and 98% respectively. RF had a sensitivity of 58% and a lower specifi city of 93 % than anti-CCP.Conclusion:
The high specifi city of the assay suggests that anti-CCP is useful in the diagnosis of rheumatoid arthritis and in our cohort of study population anti-CCP exhibits a better diagnostic value than RF. A considerable proportion (28%) of RF-negative RA patients were anti-CCP positive. Based on analytical performance of the assay, we conclude that full automation and high throughput features of AxSYM makes it an ideal platform for routine testing of anti-CCP.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
The Malaysian Journal of Pathology
Ano de publicação:
2011
Tipo de documento:
Artigo
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