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Evaluation of Direct Rapid Immunohistochemical test (DRIT) of Canis lupus familiaris hippocampal touch impression smears using a monospecific polyclonal antibody for rabies virus detection
Acta Medica Philippina ; : 51-55, 2016.
Artigo em Inglês | WPRIM | ID: wpr-632845
ABSTRACT
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BACKGROUND:

</strong> Rabies is an important zoonotic disease that needs to be eradicated worldwide. It is still prevalent in the Philippines, thus development of a relatively affordable but still accurate and rapid post-mortem detection test for the virus is desired, especially in regional laboratories.<br /><strong>

METHODS:

</strong>The study evaluated the Direct Rapid Immunohistochemical Testing (DRIT) of hippocampal touch impressions of suspected rabid Canis lupus familiaris using monospecific N protein polyclonal antibody developed by the Research Institute for Tropical Medicine (RITM). One hundred sixty (160) acetone-fixed hippocampal touch impressions were subjected DRIT.<br /><strong>

RESULTS:

</strong> One hundred thirteen (70.6%) out of 160 samples tested positive for rabies viral antigen (RVA) and 47 (29.4%) out of 160 samples tested negative for RVA. No false positive and false negative results were obtained. The results agree with the gold standard, dFAT.<br /><strong>

CONCLUSION:

</strong> DRIT was able to detect low to high concentrations of RVA in the hippocampal touch impressions based on the grading distribution. DRIT had 100% sensitivity, specificity and over-all accuracy using monospecific polyclonal antibodies, which suggests its use as a more affordable alternative to the gold standard dFAT.</p>
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Índice: WPRIM (Pacífico Ocidental) Assunto principal: Raiva / Vírus da Raiva / Tato / Medicina Tropical / Imuno-Histoquímica / Sensibilidade e Especificidade / Hipocampo / Animais / Antígenos Virais Tipo de estudo: Estudo diagnóstico Limite: Animais Idioma: Inglês Revista: Acta Medica Philippina Ano de publicação: 2016 Tipo de documento: Artigo

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Índice: WPRIM (Pacífico Ocidental) Assunto principal: Raiva / Vírus da Raiva / Tato / Medicina Tropical / Imuno-Histoquímica / Sensibilidade e Especificidade / Hipocampo / Animais / Antígenos Virais Tipo de estudo: Estudo diagnóstico Limite: Animais Idioma: Inglês Revista: Acta Medica Philippina Ano de publicação: 2016 Tipo de documento: Artigo