Protective effect of GSH on PD model induced by 6-OHDA in vitro / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 355-8, 2002.
Article
em En
| WPRIM
| ID: wpr-634113
Biblioteca responsável:
WPRO
ABSTRACT
To study the effects of 6-hydroxydopamine (6-OHDA) and reduced glutathione (GSH) on the nigral dopaminergic neurons in brain slices in vitro, immolunohistochemical technique was used to observe the changes of TH-stained neurons, including cell bodies and the dendrites, in the substantia nigra (SN) of midbrain slices of rats after incubation for 1 h in the presence of GSH 15 min before and during the period of incubation with 6-OHDA. The results showed that cell bodies remained intact but dendrites were fragmented and truncated after treatment with 6-OHDA. The antioxidant GSH alone did not significantly affect the dendrites of SN neurons but prevented 6-O-HDA-induced damage of dendrites. It was concluded that glutathione may prevent 6-OHDA-induced dopaminergic neurodegeneration and play a protective role in dopaminergic neurons.
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Índice:
WPRIM
Assunto principal:
Doença de Parkinson Secundária
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Tirosina 3-Mono-Oxigenase
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Substância Negra
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Distribuição Aleatória
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Oxidopamina
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Ratos Sprague-Dawley
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Glutationa
/
Neurônios
Tipo de estudo:
Clinical_trials
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2002
Tipo de documento:
Article