Isolation, culture and identification of neural stem cells in new-born rats / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 75-8, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-634209
ABSTRACT
The cortexes were obtained from new-born rats and dissociated to single cells by triturating. The cells were cultured in neural stem cell (NSC) culture medium (DMEM supplemented with bFGF, EGF and B27) and formed primary neurospheres after 7 days. Single cells dissociated from neurosphere were cultured in 96-well plates and formed single-cell cloning neurosphere 7 days later. The primary and single-cell cloning neurospheres were both positive for the immunofluorescent staining of nestin and were identified as NSC. It was proved that NSC can be expanded in vitro and provide seed cells for neural tissue engineering.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Separação Celular
/
Células Cultivadas
/
Córtex Cerebral
/
Ratos Sprague-Dawley
/
Meios de Cultura
/
Engenharia Tecidual
/
Animais Recém-Nascidos
/
Neurônios
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2004
Tipo de documento:
Artigo
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