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Transfection efficiency comparison of oligonucleotide and plasmid to the HL-60 cell line with liposomes / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 24-5, 2005.
Artigo em Inglês | WPRIM | ID: wpr-634220
ABSTRACT
The transfection efficiency of oligonucleotide and plasmid to the HL-60 cell line with lipofectaminePLUS was compared through observing the transfection rate and the expression duration of exogenous gene in the target cells. The results showed that the transfection rate of oligonucleotide to the HL-60 was about 90% - 95% and it had no obvious attenuation within 84 h. However, the plasmid transfection rate was only 5% -25% and it was decreased significantly within 60 h. It was suggested that the transfection of oligonucleotide with liposomes was better than that of plasmid.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oligonucleotídeos / Plasmídeos / Transfecção / Células HL-60 / Proteínas de Fluorescência Verde / Lipossomos Idioma: Inglês Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oligonucleotídeos / Plasmídeos / Transfecção / Células HL-60 / Proteínas de Fluorescência Verde / Lipossomos Idioma: Inglês Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Ano de publicação: 2005 Tipo de documento: Artigo