Use of PEI-coated magnetic iron oxide nanoparticles as gene vectors / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 618-20, 2004.
Article
em En
| WPRIM
| ID: wpr-634292
Biblioteca responsável:
WPRO
ABSTRACT
To evaluate the feasibility of using polyethyleneimine (PEI) coated magnetic iron.oxide nanoparticles (polyMAG-1000) as gene vectors. The surface characteristics of the nanoparticles were observed with scanning electron microscopy. The ability of the nanoparticles to combine with and protect DNA was investigated at different PH values after polyMAG-1000 and DNA were combined in different ratios. The nanoparticles were tested as gene vectors with in vitro transfection models. Under the scanning electron microscope the nanoparticles were about 100 nm in diameter. The nanoparticles could bind and condense DNA under acid, neutral and alkaline conditions, and they could transfer genes into cells and express green fluorescent proteins (GFP). The transfection efficiency was highest (51%) when the ratio of nanoparticles to DNA was 1:1 (v:w). In that ratio, the difference in transfection efficiency was marked depending on whether a magnetic field was present or not: about 10% when it was absent but 51% when it was present. The magnetic iron oxide nanoparticles coated with PEI may potentially be used as gene vectors.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Tamanho da Partícula
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Polietilenoimina
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Neoplasias da Mama
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Compostos Férricos
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Transfecção
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Marcação de Genes
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Nanotecnologia
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Linhagem Celular Tumoral
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Proteínas de Fluorescência Verde
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Vetores Genéticos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2004
Tipo de documento:
Article