Up-regulation of prothymosin alpha in THP-1 cells infected with Mycobacterium tuberculosis
Journal of the Korean Society for Microbiology
; : 149-157, 2000.
Article
em Ko
| WPRIM
| ID: wpr-63568
Biblioteca responsável:
WPRO
ABSTRACT
Mycobacterium tuberculosis is capable of growing and survival within macrophage. The purpose of this study was to identify the genes regulated by infection of mycobacteria in human monocytic THP-1 cells. We used the differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) and nothern blot analysis to confirm the differentially expressed genes from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv, heat-kille Mycobacterium tuberculosis H37Rv and live Mycobacterium bovis BCG. Among many up or down-regulated clones, 27 clones were sequenced and compared with known genes on GenBank. Thirteen of over-expressed clones from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv were identical to human prothymosin alpha, eight were novel clones and six clones showed homology with Human ferritin H chain, Escherichia coli bgl, Mouse RNA-dependent EIF-2 alpha kinase, E. coli htrL, Hyaluronan receptor and T cell receptor. Our result suggests that Mycobacterium tuberculosis might regulate prothymosin alpha gene transcription in monocytic THP-1 cell.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosfotransferases
/
Receptores de Antígenos de Linfócitos T
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Fator de Iniciação 2 em Eucariotos
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Regulação para Cima
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Células Clonais
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Receptores de Hialuronatos
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Bases de Dados de Ácidos Nucleicos
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Escherichia coli
/
Ferritinas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Ko
Revista:
Journal of the Korean Society for Microbiology
Ano de publicação:
2000
Tipo de documento:
Article