Quick identification of haemophilus influenza with reverse dot blot / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
;
(24)1992.
Artigo
em Chinês
| WPRIM
| ID: wpr-638322
ABSTRACT
Objective To study the way of quick identification of haemophilus influenzae with reverse dot blot.Methods Oligonucleotide probe which is specially targeted to 16SrDNA of haemophilus influenzae was designed, then fixed the probe to nylon membranes, and hybridized with the production of gain with the universal primers.Results The universal primers could hybridize the target sequence from common pathogenic bacteria by PCR, and oligonucleotide probe could hybridize with haemophilus influenza specially and could not hybridize with other bacterias. It proved that the probe was of highly speciality.Conclusion Reverse dot blot is a good method of quickly identification of haemophilus influenzae.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Applied Clinical Pediatrics
Ano de publicação:
1992
Tipo de documento:
Artigo
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