Influence of Carbon Monoxide on the Content of Atrial Natriuretic Peptide in Ventricular Tissues of Rats / 实用儿科临床杂志

ABSTRACT

Objective To investigate the influence of carbon monoxide(CO) on the content of atrial natriuretic peptide(ANP) in ventricular tissues of rats.Methods The left and right ventricular tissues of 12 Wistar rats were divided into control group(n=12) and hemin group(n=12,the final concentration of hemin was 10~(-4) mol/L),respectively.The ventricular tissues were successly incubated in(37 ℃) thermostatic waterbath with Krebs solution as supernatant for 4 hours.After incubation of the ventricular tissues,radio-immunity method was applied to determine the content of ANP in the supernatant.Results Compared with the content of ANP in the supernatant in control group,it was significantly decreased both in left [(9.72?3.59) vs(52.05?31.65) ng/(g?wet tissue),P