Immunohistochemical Expression of Hirschsprung′s Disease / 实用儿科临床杂志

ABSTRACT

Objective To investigate the expressions of protein gene product 9.5 (PGP 9.5),cathepsin D(CAD) and S-100 protein(S100) and their significance in the colon of the patients with hirschsprung′s disease(HD).Methods Thirty stenotic segments from children with HD and 35 controls were stained for PGP 9.5 and CAD and S100 by immunohistochemistry.The expressions were observed by imaging analysis system.Results 1.In the controls,CAD was only positive to ganglionic cells,which could be observed in myenteric and submucosal plexuses.Schwann cells and perineural satellite cells and nerve fibers were positive to PGP 9.5 and S100,ganglionic cells were positive to PGP 9.5,and were stained negative to S100,which left "cells-like blank area" in the plexuses.2.In the stenotic segments of HD,neither CAD-positive and PGP 9.5-positive neurons nor "cell-like blank area" in S100 staining were found in the plexus.The PGP 9.5 and S100 positive fibers were proliferated obviously in number.There were obvious differences in the density of the S100 stained nerve fibers and the fiber sizes between the controls[169.25?9.53;(23.15?5.56) ?m ] and the stenotic segments of HD[146.70?10.87;(57.81?14.99) ?m](all P